PoplarV1, Main, Exploration, bibRecord, 000D17

Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa.

Identifieur interne : 000D17 ( Main/Exploration ); précédent : 000D16; suivant : 000D18

Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa.

Auteurs : Liuqiang Wang [République populaire de Chine] ; Xiaoling Zhang [République populaire de Chine] ; Jin Zhang [République populaire de Chine] ; Wei Fan [République populaire de Chine] ; Mengzhu Lu [République populaire de Chine] ; Jianjun Hu [République populaire de Chine]

Source :

International journal of molecular sciences [ 1422-0067 ] ; 2018.

RBID : pubmed:29337913

Descripteurs français

KwdFr :
- Allergènes (métabolisme), Analyse de profil d'expression de gènes (MeSH), Annotation de séquence moléculaire (MeSH), Gene Ontology (MeSH), Germination (MeSH), Pollen (croissance et développement), Pollen (métabolisme), Populus (génétique), Populus (métabolisme), Protéines végétales (métabolisme), Protéomique (méthodes), Régulation de l'expression des gènes végétaux (MeSH), Spécificité d'organe (MeSH), Électrophorèse bidimensionnelle sur gel (MeSH).
MESH :
- croissance et développement : Pollen.
- génétique : Populus.
- métabolisme : Allergènes, Pollen, Populus, Protéines végétales.
- méthodes : Protéomique.
- Analyse de profil d'expression de gènes, Annotation de séquence moléculaire, Gene Ontology, Germination, Régulation de l'expression des gènes végétaux, Spécificité d'organe, Électrophorèse bidimensionnelle sur gel.

English descriptors

KwdEn :
- Allergens (metabolism), Electrophoresis, Gel, Two-Dimensional (MeSH), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Gene Ontology (MeSH), Germination (MeSH), Molecular Sequence Annotation (MeSH), Organ Specificity (MeSH), Plant Proteins (metabolism), Pollen (growth & development), Pollen (metabolism), Populus (genetics), Populus (metabolism), Proteomics (methods).
MESH :
- chemical , metabolism : Allergens, Plant Proteins.
- genetics : Populus.
- growth & development : Pollen.
- metabolism : Pollen, Populus.
- methods : Proteomics.
- Electrophoresis, Gel, Two-Dimensional, Gene Expression Profiling, Gene Expression Regulation, Plant, Gene Ontology, Germination, Molecular Sequence Annotation, Organ Specificity.

Abstract

Pollen grains from Populus tomentosa, a widely cultivated tree in northern area of China, are considered to be an important aeroallergen causing severe allergic diseases. To gain insight into their allergenic components, mature Populus tomentosa pollen proteins were analyzed by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS). A total of 412 spots from mature pollen were resolved on pH 4-7 immobilized pH gradient (IPG) strips and 159 distinct proteins were identified from 242 spots analyzed. The identified proteins were categorized based on their functional role in the pollen, which included proteins involved in energy regulation, protein fate, protein synthesis and processing, metabolism, defense/stress responses, development and other functional categories. Moreover, among the identified proteins, 27 proteins were identified as putative allergens using the Structural Database of Allergenic Proteins (SDAP) tool and Allergen Online. The expression patterns of these putative allergen genes indicate that several of these genes are highly expressed in pollen. The identified putative allergens have the potential to improve specific diagnosis and can be used to develop vaccines for immunotherapy against poplar pollen allergy.

DOI: 10.3390/ijms19010250
PubMed: 29337913
PubMed Central: PMC5796197

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<term>Electrophoresis, Gel, Two-Dimensional (MeSH)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Gene Ontology (MeSH)</term>
<term>Germination (MeSH)</term>
<term>Molecular Sequence Annotation (MeSH)</term>
<term>Organ Specificity (MeSH)</term>
<term>Plant Proteins (metabolism)</term>
<term>Pollen (growth & development)</term>
<term>Pollen (metabolism)</term>
<term>Populus (genetics)</term>
<term>Populus (metabolism)</term>
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<term>Annotation de séquence moléculaire (MeSH)</term>
<term>Gene Ontology (MeSH)</term>
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<term>Pollen (croissance et développement)</term>
<term>Pollen (métabolisme)</term>
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<front><div type="abstract" xml:lang="en">Pollen grains from <i>Populus tomentosa</i>
, a widely cultivated tree in northern area of China, are considered to be an important aeroallergen causing severe allergic diseases. To gain insight into their allergenic components, mature <i>Populus tomentosa</i>
 pollen proteins were analyzed by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS). A total of 412 spots from mature pollen were resolved on pH 4-7 immobilized pH gradient (IPG) strips and 159 distinct proteins were identified from 242 spots analyzed. The identified proteins were categorized based on their functional role in the pollen, which included proteins involved in energy regulation, protein fate, protein synthesis and processing, metabolism, defense/stress responses, development and other functional categories. Moreover, among the identified proteins, 27 proteins were identified as putative allergens using the Structural Database of Allergenic Proteins (SDAP) tool and Allergen Online. The expression patterns of these putative allergen genes indicate that several of these genes are highly expressed in pollen. The identified putative allergens have the potential to improve specific diagnosis and can be used to develop vaccines for immunotherapy against poplar pollen allergy.</div>
</front>
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<DateCompleted><Year>2018</Year>
<Month>08</Month>
<Day>03</Day>
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<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
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<Issue>1</Issue>
<PubDate><Year>2018</Year>
<Month>Jan</Month>
<Day>16</Day>
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<Title>International journal of molecular sciences</Title>
<ISOAbbreviation>Int J Mol Sci</ISOAbbreviation>
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<ArticleTitle>Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa.</ArticleTitle>
<ELocationID EIdType="pii" ValidYN="Y">E250</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.3390/ijms19010250</ELocationID>
<Abstract><AbstractText>Pollen grains from <i>Populus tomentosa</i>
, a widely cultivated tree in northern area of China, are considered to be an important aeroallergen causing severe allergic diseases. To gain insight into their allergenic components, mature <i>Populus tomentosa</i>
 pollen proteins were analyzed by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS). A total of 412 spots from mature pollen were resolved on pH 4-7 immobilized pH gradient (IPG) strips and 159 distinct proteins were identified from 242 spots analyzed. The identified proteins were categorized based on their functional role in the pollen, which included proteins involved in energy regulation, protein fate, protein synthesis and processing, metabolism, defense/stress responses, development and other functional categories. Moreover, among the identified proteins, 27 proteins were identified as putative allergens using the Structural Database of Allergenic Proteins (SDAP) tool and Allergen Online. The expression patterns of these putative allergen genes indicate that several of these genes are highly expressed in pollen. The identified putative allergens have the potential to improve specific diagnosis and can be used to develop vaccines for immunotherapy against poplar pollen allergy.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Liuqiang</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China. wanglq@caf.ac.cn.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China. wanglq@caf.ac.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Xiaoling</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China. xlzhang@126.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Jin</ForeName>
<Initials>J</Initials>
<Identifier Source="ORCID">0000-0002-8397-5078</Identifier>
<AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China. zhang007jin@163.com.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Fan</LastName>
<ForeName>Wei</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China. atom201001@163.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lu</LastName>
<ForeName>Mengzhu</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China. lumz@caf.ac.cn.</Affiliation>
</AffiliationInfo>
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<Initials>J</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China. hujj@caf.ac.cn.</Affiliation>
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<affiliations><list><country><li>République populaire de Chine</li>
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<tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Liuqiang" sort="Wang, Liuqiang" uniqKey="Wang L" first="Liuqiang" last="Wang">Liuqiang Wang</name>
</noRegion>
<name sortKey="Fan, Wei" sort="Fan, Wei" uniqKey="Fan W" first="Wei" last="Fan">Wei Fan</name>
<name sortKey="Hu, Jianjun" sort="Hu, Jianjun" uniqKey="Hu J" first="Jianjun" last="Hu">Jianjun Hu</name>
<name sortKey="Hu, Jianjun" sort="Hu, Jianjun" uniqKey="Hu J" first="Jianjun" last="Hu">Jianjun Hu</name>
<name sortKey="Lu, Mengzhu" sort="Lu, Mengzhu" uniqKey="Lu M" first="Mengzhu" last="Lu">Mengzhu Lu</name>
<name sortKey="Lu, Mengzhu" sort="Lu, Mengzhu" uniqKey="Lu M" first="Mengzhu" last="Lu">Mengzhu Lu</name>
<name sortKey="Wang, Liuqiang" sort="Wang, Liuqiang" uniqKey="Wang L" first="Liuqiang" last="Wang">Liuqiang Wang</name>
<name sortKey="Zhang, Jin" sort="Zhang, Jin" uniqKey="Zhang J" first="Jin" last="Zhang">Jin Zhang</name>
<name sortKey="Zhang, Xiaoling" sort="Zhang, Xiaoling" uniqKey="Zhang X" first="Xiaoling" last="Zhang">Xiaoling Zhang</name>
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Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa.

Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa.

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